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Nghiên cứu định lượng hợp chất sarcophine từ cao chiết loài san hô mềm Sarcophyton ehrenbergi bằng phương pháp HPLC-DAD
Vũ Anh Tú1, Châu Ngọc Điệp, Ninh Thị Ngọc1, Cao Ngọc Anh2, Đoàn Cao Sơn2, Nguyễn Văn Thanh1, Nguyễn Xuân Cường1, Nguyễn Hoài Nam1, Châu Văn Minh1
1Viện Hóa sinh biển (IMBC), Viện Hàn lâm Khoa học và Công nghệ Việt Nam (VAST)
2Viện Kiểm nghiệm thuốc Trung ương
High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for the quantitative determination of sarcophine (1) in methanol extract of the soft coral Sarcophyton ehrenbergi. The separations were effected by using a mobile phase of acetonitrile and water. The gradient elution of the mobile phase was 50-95% ACN in 0-25 min, 95% ACN in 25-35 min. The flow rate was 0.8 ml/min and the detector wavelength was set at 210 nm. This method was validated in terms of compatibility, specificity, linearity, precision, accuracy, limit of detection and limit of quantitation.
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