Phương pháp định lượng hợp chất 3,5-dibromo-1-hydroxy-4,4-dimethoxy-2,5 cyclohexadiene-1-acetamide từ cao chiết loài hải miên Acanthella obtusa bằng HPLC-DAD
Vũ Anh Tú1, Châu Ngọc Điệp1, Nguyễn Thị Cúc1, Đan Thị Thúy Hằng1, Hoàng Lê Tuấn Anh1, Đinh Thị Nhung2, Nguyễn Thị Ánh Hường2, Nguyễn Văn Thanh1,
Nguyễn Xuân Cường1, Nguyễn Hoài Nam1, Châu Văn Minh1
1Viện Hóa sinh biển (IMBC), Viện Hàn lâm Khoa học và Công nghệ Việt Nam (VAST)
2Trường Đại học Khoa học Tự nhiên, Đại học Quốc gia Hà Nội
High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for the quantitative determination of 3,5-dibromo-1-hydroxy-4,4-dimethoxy-2,5 cyclohexadiene-1-acetamide (1) in methanol extract of the sponge Acanthella obtusa. The separations were effected by using a mobile phase of acetonitrile and water. The gradient elution of the mobile phase was 5-20% ACN in 0-12 min, 20-60% ACN in 12-50 min, 60-75% ACN in 50-65 min, 75-95% ACN in 65-95 min. The flow rate was 0.5 ml/min and the detector wavelength was set at 210 nm. This method was validated in terms of compatibility, specificity, linearity, precision, accuracy, limit of detection and limit of quantitation.
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