Phương pháp định lượng hợp chất O-methyl furodysinin lactone từ cao chiết loài hải miên Dysidea fragilis (Montagu, 1818) bằng HPLC-DAD
Vũ Anh Tú, Châu Ngọc Điệp, Nguyễn Thị Cúc, Đan Thị Thúy Hằng, Hoàng Lê Tuấn Anh, Nguyễn Văn Thanh, Nguyễn Xuân Cường, Nguyễn Hoài Nam, Châu Văn Minh
Viện Hóa sinh biển (IMBC), Viện Hàn lâm Khoa học và Công nghệ Việt Nam (VAST)
High performance liquid chromatography with diode array detector (HPLC-DAD) was developed for the quantitative determination of O-methyl furodysinin lactone (1) in methanol extract of the sponge Dysidea fragilis (Montagu, 1818). The separations were effected by using a mobile phase of acetonitrile and water. The gradient elution of the mobile phase was 5-20% ACN in 0-12 min, 20-60% ACN in 12-50 min, 60-75% ACN in 50-65 min, 75-95% ACN in 65-95 min. The flow rate was 0.5 ml/min and the detector wavelength was set at 210 nm. This method was validated in terms of compatibility, specificity, linearity, precision, accuracy, limit of detection and limit of quantitation.
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