Figure 7. Effects of Aspergillus oryzae and Aspergillus niger co-cultivation fermentation extract on the composition of phyla
of bacterial communities in a TMR (B), corn silage (C), oat hay (D), alfalfa hay (E), and all (A). AOAN indicates fermenta-
tion with Aspergillus oryzae and Aspergillus niger co-cultivation fermentation extract. CON group indicates all feeds without
AOAN supplementation. AOAN group indicates all feeds with AOAN supplementation. TMR, total mixed ration.
3.4.3. Differential Genera
At the genus level, the top 20 differential genera between two different groups were
examined by |LDA| score > 2 and adjusted-p < 0.10 (Figure 8). There were no significant
differences in the corn silage group with AOAN supplementation (p > 0.10, |LDA| score
< 2). Prevotella_1 belonging to Bacteroidetes exhibited higher relative abundance following
AOAN supplementation (Figure 8A–D; p < 0.10). The genera Prevotellaceae_UCG-003 and
Prevotellaceae_UCG-001 belonging to Bacteroidetes in CON, TMR, and alfalfa hay (Figure
8A,B,D), and Selenomonas_1 and Anaerovibrio belonging to Firmicutes in CON, oat hay,
and alfalfa hay (Figure 8A,C,D) were increased (p < 0.10). The genera Syntrophococcus, Ru-
minococcaceae_UCG-013, Lachnospira, and Butyrivibrio_2 belonging to Firmicutes in CON,
oat hay, and alfalfa hay were decreased by AOAN supplementation (Figure 8A,C,D; p <
0.10).
Figure 7.
Effects of Aspergillus oryzae and Aspergillus niger co-cultivation fermentation extract on the composition of phyla of
bacterial communities in a TMR (B), corn silage (C), oat hay (D), alfalfa hay (E), and all (A). AOAN indicates fermentation
with Aspergillus oryzae and Aspergillus niger co-cultivation fermentation extract. CON group indicates all feeds without
AOAN supplementation. AOAN group indicates all feeds with AOAN supplementation. TMR, total mixed ration.
3.4.3. Differential Genera
At the genus level, the top 20 differential genera between two different groups were
examined by |LDA| score > 2 and adjusted-p < 0.10 (Figure
8
). There were no significant
differences in the corn silage group with AOAN supplementation (p > 0.10, |LDA| score
< 2). Prevotella_1 belonging to Bacteroidetes exhibited higher relative abundance follow-
ing AOAN supplementation (Figure
8
A–D; p < 0.10). The genera Prevotellaceae_UCG-003
and Prevotellaceae_UCG-001 belonging to Bacteroidetes in CON, TMR, and alfalfa hay
(Figure
8
A,B,D), and Selenomonas_1 and Anaerovibrio belonging to Firmicutes in CON, oat
hay, and alfalfa hay (Figure
8
A,C,D) were increased (p < 0.10). The genera Syntrophococ-
cus, Ruminococcaceae_UCG-013, Lachnospira, and Butyrivibrio_2 belonging to Firmicutes in
CON, oat hay, and alfalfa hay were decreased by AOAN supplementation (Figure
8
A,C,D;
p < 0.10).
Animals 2021, 11, 1248
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Animals 2021, 11, x
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