| Figure 8.
Differential bacterial genera between CON (all feeds without AOAN supplementation) and AOAN (all feeds with
AOAN supplementation) (A), TMR and TMR + AOAN (B), oat hay and oat hay + AOAN (C), alfalfa hay and alfalfa hay +
AOAN (D). AOAN indicated feed fermentation with Aspergillus oryzae and Aspergillus niger co-cultivation fermentation
extract. CON group indicates all feeds without AOAN supplementation. AOAN group indicates all feeds with AOAN
supplementation. Significant differences were tested by linear discriminant analysis effect size (LEfSe) and declared as
|linear discriminant analysis score| > 2 and adjusted-p < 0.10. The genera in red indicate that the genus was enriched more
than three times. TMR, total mixed ration.
3.4.4. Spearman Correlation Analysis
Spearman correlation analysis between the relative abundance of genus features from
AOAN vs. CON and phenotypic indices is shown in Figure
9
. The results show that the
relative abundance of Prevotella_1, Prevotella_UCG_001, and Anaerovibrio were positively
correlated with DM digestibility, TVFA, and molar proportion of acetate (p < 0.05), and neg-
atively correlated with the molar proportion of butyrate (p < 0.05). The relative abundance
of Prevotella_UCG_003 and Prevotella_UCG_001 were positively correlated with acetate to
Animals 2021, 11, 1248
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propionate ratio (p < 0.05). There was a significant negative correlation between DM di-
gestibility, TVFA, and the relative abundance of Syntrophococcus, Ruminococcaceae_UCG_013,
Butyrivibrio_2, and Lachnospira (p < 0.05).
Animals 2021, 11, x
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between DM digestibility, TVFA, and the relative abundance of Syntrophococcus, Rumino-
coccaceae_UCG_013, Butyrivibrio_2, and Lachnospira ( p < 0.05).
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