2.1. Animals
Three multiparous, rumen-cannulated, lactating, Holstein dairy cows (550
±
25.4 kg
9 days in milk; 32
2.67 kg/d milk yield; mean
standard deviation)
Beijing. The main ingredients and chemical composition of the TMR are shown in Table
1
. The cows were fed three times daily at 0730, 1330, and 1830, and milked three times daily
Care and Use Committee of the China Agricultural University (CAU20201009-2).
3 of 19
Ingredients of total mixed ration used in vitro (dry matter basis).