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Chen
et al. Vet Res (2021) 52:73
(Addgene, #12259) to obtain recombinant lentiviruses
at 72 h. Then, the culture
supernatant was collected
and used to infect PK-15 cells. After 48 h, the cells were
selected by puromycin at a concentration of 10 μg/mL.
Positive cells were obtained after about 2 weeks and then
subcloned into 96-well plates
for single-clone growth and
saved as cell stocks.
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