Syncrip facilitates porcine parvovirus viral dna replication through the alternative splicing of ns1 mrna to promote ns2 mrna formation
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- Introduction
| Keywords: PPV, SYNCRIP, NS1 mRNA, Alternative splicing, NS2 mRNA
© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/ . The Creative Commons Public Domain Dedication waiver ( http:// creat iveco mmons. org/ publi cdoma in/ zero/1. 0/ ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Introduction Porcine Parvovirus (PPV) is a major causative agent of stillbirth, mummification, and embryonic death in swine [ 1 ]. PPV is a single negative strand DNA virus that belongs to the species ungulate protoparvovirus 1 in the genus protoparvovirus [ 2 ], which contains two identical inverted terminal repeats (ITR) at both ends (Figure 1 A). The genome contains two major open reading frames (ORF), the ORF1 encodes the nonstructural proteins NS1, NS2 and NS3, whereas the ORF2 encodes struc- tural proteins VP1, VP2 and nonstructural protein SAT (Figures 1 B, C). NS1 is a multifunctional protein, which is necessary for effective viral replication and production of infective virion [ 3 , 4 ]. It is related to cell apoptosis induc- tion [ 5 , 6 ], cell cycle arrest and the suppression of type I interferon responses [ 7 , 8 ]. Mature NS2 mRNA is com- pletely located in NS1 mRNA, which is formed by NS1 mRNA after alternative splicing. During the life cycle of virus, it is very important that the ratio of NS2 to NS1 is precisely regulated. PPV NS2 protein suppresses type I interferon responses [ 9 ] and participates in viral egress from the nucleus by interacting with nuclear export fac- tor CRM1 [ 10 ]. However, how NS1 mRNA is spliced to form NS2 mRNA is unclear. SYNCRIP is a member of heterogeneous nuclear ribonucleoproteins (hnRNP), also known as NS1 tải về 5.65 Mb. Chia sẻ với bạn bè của bạn:
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