Chen
et al. Vet Res (2021) 52:73
https://doi.org/10.1186/s13567-021-00938-6
RESEARCH ARTICLE
SYNCRIP facilitates porcine parvovirus viral
DNA replication through the alternative splicing
of NS1 mRNA to promote NS2
mRNA formation
Songbiao Chen
†
, Bichen Miao
†
, Nannan Chen,
Caiyi Chen, Ting Shao, Xuezhi Zhang, Lingling Chang,
Xiujuan Zhang, Qian Du, Yong Huang
*
and
Dewen Tong
*
Abstract
Porcine Parvovirus (PPV), a pathogen causing porcine reproductive disorders, encodes two capsid proteins (VP1 and
VP2) and three nonstructural proteins (NS1, NS2 and SAT) in infected cells. The PPV NS2 mRNA is from NS1 mRNA after
alternative splicing, yet the corresponding mechanism is unclear.
In this study, we identified a PPV NS1 mRNA bind-
ing protein SYNCRIP, which belongs to the hnRNP family and has been identified to be
involved in host pre-mRNA
splicing by RNA-pulldown and mass spectrometry approaches. SYNCRIP was found to be significantly up-regulated
by PPV infection in vivo and in vitro. We confirmed that it directly interacts with PPV NS1 mRNA and is co-localized
at the cytoplasm in PPV-infected cells. Overexpression of SYNCRIP significantly reduced the NS1
mRNA and protein
levels, whereas deletion of SYNCRIP significantly reduced NS2 mRNA and protein levels and the ratio of NS2 to NS1,
and further impaired replication of the PPV. Furthermore, we found that SYNCRIP was able to bind the 3
′-terminal site
of NS1 mRNA to promote the cleavage of NS1 mRNA into NS2 mRNA. Taken together, the
results presented here dem-
onstrate that SYNCRIP is a critical molecule in the alternative splicing process of PPV mRNA, while revealing a novel
function for this protein and providing a potential target of antiviral intervention for the control of porcine parvovirus
disease.