PHỤ LỤC
Phụ lục 1: Danh sách mẫu mô của bệnh nhân ung thư gan nguyên phát (HCC) cùng với mẫu mô lành cách khối u 5cm sử dụng trong nghiên cứu
-
STT
|
Họ và tên
|
Mã
|
Tuổi
|
Giới tính
|
Chẩn đoán
|
Ngày mổ
|
1
|
Hoàng Thị M
|
X9634
|
60
|
Nữ
|
HCC
|
7-10-2009
|
2
|
Bùi Văn T
|
X7456
|
40
|
Nam
|
HCC
|
24-08-2009
|
3
|
Bùi Minh T
|
X9242
|
50
|
Nam
|
HCC
|
1-10-2009
|
4
|
Nguyễn Thị N
|
Y908
|
53
|
Nữ
|
HCC
|
02-12-2009
|
5
|
Phạm Thị P
|
8529
|
69
|
Nữ
|
HCC
|
12-04-2009
|
6
|
Lê Văn S
|
8702
|
31
|
Nam
|
HCC
|
12-04-2009
|
7
|
Trần Văn C
|
7632
|
43
|
Nam
|
HCC
|
12-04-2009
|
Phụ lục 2: Điện di hai chiều mẫu protein ty thể và microsome tách từ mô gan
Chiều 1: sợi IPG strip pH 4-7 dài 17cm; Chiều 2: gel polyacrylamide 10% có SDS (SDS-PAGE)
|
Bệnh nhân: Y908: (A). Mô đối chứng (B). Mô ung thư
|
|
Bệnh nhân: X9242: (A). Mô đối chứng (B). Mô ung thư
|
|
Bệnh nhân: X7456: (A). Mô đối chứng (B). Mô ung thư
|
|
Bệnh nhân: X9634: (A). Mô đối chứng (B). Mô ung thư
|
|
Bệnh nhân: 8529: (A). Mô đối chứng (B). Mô ung thư
|
|
Bệnh nhân: 7632: (A). Mô đối chứng (B). Mô ung thư
|
Phụ lục 3: Kết quả nhận dạng protein bằng cơ sở dữ liệu NCBI sử dụng phần mềm Mascot
Mascot Search Results
User : Mai Thi Hồng
Email : maihonghong@gmail.com
Search title :
Database : MSDB 20060831 (3239079 sequences; 1079594700 residues)
Taxonomy : Homo sapiens (human) (148148 sequences)
Timestamp : 24 Mar 2010 at 15:45:57 GMT
Top Score : 88 for Q53HF2_HUMAN, Heat shock 70kDa protein 8 isoform 2 variant (Fragment).- Homo sapiens (Human).
Mascot Score Histogram
Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 64 are significant (p<0.05).
Trên cùng của Biểu mẫu
Concise Protein Summary Report
Đáy của Biểu mẫu
Trên cùng của Biểu mẫu
1.
|
MVP_HUMAN Mass: 99135 Score: 74 Expect: 0.0055 Matches: 9
|
|
Major vault protein (MVP) (Lung resistance-related protein).- Homo sapiens (Human).
|
|
AAH15623 Mass: 99266 Score: 74 Expect: 0.0056 Matches: 9
|
|
BC015623 NID: - Homo sapiens
|
|
S57723 Mass: 99736 Score: 74 Expect: 0.0056 Matches: 9
|
|
lrp protein – human
|
|
Q96HX3_HUMAN Mass: 64542 Score: 40 Expect: 14 Matches: 5
|
|
Similar to ribophorin I (Fragment).- Homo sapiens (Human).
|
|
A26168 Mass: 68527 Score: 39 Expect: 18 Matches: 5
|
|
ribophorin I precursor - human
|
|
Q53EP4_HUMAN Mass: 68537 Score: 39 Expect: 18 Matches: 5
|
|
Ribophorin I variant (Fragment).- Homo sapiens (Human).
|
|
Q3SXM8_HUMAN Mass: 3766 Score: 33 Expect: 81 Matches: 2
|
|
GPR110 protein.- Homo sapiens (Human).
|
|
Q6IBR0_HUMAN Mass: 68564 Score: 32 Expect: 89 Matches: 4
|
|
RPN1 protein.- Homo sapiens (Human).
|
|
CAC38568 Mass: 26058 Score: 32 Expect: 91 Matches: 3
|
|
Sequence 153 from Patent WO0129221.- Homo sapiens (Human).
|
|
Q96H35_HUMAN Mass: 21635 Score: 32 Expect: 1e+02 Matches: 3
|
|
RNA binding motif protein 18 (OTTHUMP00000022049).- Homo sapiens (Human).
|
|
AAF06940 Mass: 28286 Score: 31 Expect: 1.2e+02 Matches: 3
|
|
AF126781 NID: - Homo sapiens
|
|
AAQ88796 Mass: 28300 Score: 31 Expect: 1.2e+02 Matches: 3
|
|
AY358430 NID: - Homo sapiens
|
|
T44603 Mass: 32785 Score: 30 Expect: 1.4e+02 Matches: 4
|
|
hypothetical protein CGI-83 [imported] - human
|
|
Q53H82_HUMAN Mass: 32769 Score: 30 Expect: 1.4e+02 Matches: 4
|
|
Lactamase, beta 2 variant (Fragment).- Homo sapiens (Human).
|
|
Q50EM8_HUMAN Mass: 5407 Score: 30 Expect: 1.6e+02 Matches: 2
|
|
CC chemokine ligand 4L1d2.- Homo sapiens (Human).
|
|
Q7YQH3_PANTR Mass: 16378 Score: 30 Expect: 1.6e+02 Matches: 3
|
|
GDP dissociation inhibitor 1 (Fragment).- Pan troglodytes verus.
|
|
Q9BX99_HUMAN Mass: 79512 Score: 30 Expect: 1.7e+02 Matches: 5
|
|
Mutant early onset breast cancer susceptibility protein 2 (Fragment).- Homo sapiens (Human).
|
|
AAG32314 Mass: 45363 Score: 29 Expect: 1.8e+02 Matches: 4
|
|
AF287303 NID: - Homo sapiens
|
|
AAH12779 Mass: 45362 Score: 29 Expect: 1.8e+02 Matches: 4
|
|
BC012779 NID: - Homo sapiens
|
|
AAM88116 Mass: 7320 Score: 28 Expect: 2.1e+02 Matches: 2
|
|
Immunoglobulin heavy chain variable region (Fragment).- Homo sapiens (Human).
|
|
Q8ND89_HUMAN Mass: 25467 Score: 28 Expect: 2.2e+02 Matches: 3
|
|
Hypothetical protein DKFZp434O131.- Homo sapiens (Human).
|
|
AAD16714 Mass: 10647 Score: 28 Expect: 2.2e+02 Matches: 2
|
|
IMMUNOGLOBULIN LAMBDA LIGHT CHAIN VARIABLE REGION (FRAGMENT).- Homo sapiens (Human).
|
|
CAA85590 Mass: 13372 Score: 28 Expect: 2.5e+02 Matches: 2
|
|
IMMUNOGLOBULIN KAPPA LIGHT CHAIN VARIABLE REGION (FRAGMENT).- Homo sapiens (Human).
|
2.
|
CAD88647 Mass: 12812 Score: 37 Expect: 32 Matches: 3
|
|
Immunoglobulin heavy chain (Fragment).- Homo sapiens (Human).
|
|
CAF14673 Mass: 6346 Score: 28 Expect: 2.3e+02 Matches: 2
|
|
AX969565 NID: - Homo sapiens
|
Đáy của Biểu mẫu
Search Parameters
Type of search : Peptide Mass Fingerprint
Enzyme : Trypsin
Mass values : Monoisotopic
Protein Mass : Unrestricted
Peptide Mass Tolerance : ± 1 Da
Peptide Charge State : 1+
Max Missed Cleavages : 1
Number of queries : 11
Mascot: http://www.matrixscience.com/
|
Mascot Search Results
Protein View
Trên cùng của Biểu mẫu
Match to: MVP_HUMAN Score: 74 Expect: 0.0055
Major vault protein (MVP) (Lung resistance-related protein).- Homo sapiens (Human).
Nominal mass (Mr): 99135; Calculated pI value: 5.34
NCBI BLAST search of MVP_HUMAN against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Homo sapiens
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 11
Number of mass values matched: 9
Sequence Coverage: 15%
Matched peptides shown in Bold Red
1 ATEEFIIRIP PYHYIHVLDQ NSNVSRVEVG PKTYIRQDNE RVLFAPMRMV
51 TVPPRHYCTV ANPVSRDAQG LVLFDVTGQV RLRHADLEIR LAQDPFPLYP
101 GEVLEKDITP LQVVLPNTAL HLKALLDFED KDGDKVVAGD EWLFEGPGTY
151 IPRKEVEVVE IIQATIIRQN QALRLRARKE CWDRDGKERV TGEEWLVTTV
201 GAYLPAVFEE VLDLVDAVIL TEKTALHLRA RRNFRDFRGV SRRTGEEWLV
251 TVQDTEAHVP DVHEEVLGVV PITTLGPHNY CVILDPVGPD GKNQLGQKRV
301 VKGEKSFFLQ PGEQLEQGIQ DVYVLSEQQG LLLRALQPLE EGEDEEKVSH
351 QAGDHWLIRG PLEYVPSAKV EVVEERQAIP LDENEGIYVQ DVKTGKVRAV
401 IGSTYMLTQD EVLWEKELPP GVEELLNKGQ DPLADRGEKD TAKSLQPLAP
451 RNKTRVVSYR VPHNAAVQVY DYREKRARVV FGPELVSLGP EEQFTVLSLS
501 AGRPKRPHAR RALCLLLGPD FFTDVITIET ADHARLQLQL AYNWHFEVND
551 RKDPQETAKL FSVPDFVGDA CKAIASRVRG AVASVTFDDF HKNSARIIRT
601 AVFGFETSEA KGPDGMALPR PRDQAVFPQN GLVVSSVDVQ SVEPVDQRTR
651 DALQRSVQLA IEITTNSQEA AAKHEAQRLE QEARGRLERQ KILDQSEAEK
701 ARKELLELEA LSMAVESTGT AKAEAESRAE AARIEGEGSV LQAKLKAQAL
751 AIETEAELQR VQKVRELELV YARAQLELEV SKAQQLAEVE VKKFKQMTEA
801 IGPSTIRDLA VAGPEMQVKL LQSLGLKSTL ITDGSTPINL FNTAFGLLGM
851 GPEGQPLGRR VASGPSPGEG ISPQSAQAPQ APGDNHVVPV LR
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) Delta Miss Sequence
9 - 26 2151.7000 2150.6927 2151.0861 -0.3934 0 R.IPPYHYIHVLDQNSNVSR.V
67 - 81 1617.7000 1616.6927 1616.8522 -0.1595 0 R.DAQGLVLFDVTGQVR.L
233 - 238 854.5000 853.4927 853.4195 0.0732 1 R.NFRDFR.G
293 - 299 842.6000 841.5927 842.4722 -0.8795 1 K.NQLGQKR.V
454 - 460 880.5000 879.4927 879.4926 0.0001 1 K.TRVVSYR.V
461 - 473 1531.6000 1530.5927 1530.7579 -0.1652 0 R.VPHNAAVQVYDYR.E
479 - 505 2856.7000 2855.6927 2855.5433 0.1494 0 R.VVFGPELVSLGPEEQFTVLSLSAGRPK.R
623 - 648 2812.6000 2811.5927 2811.4039 0.1888 0 R.DQAVFPQNGLVVSSVDVQSVEPVDQR.T
745 - 760 1783.8000 1782.7927 1782.9839 -0.1912 1 K.LKAQALAIETEAELQR.V
No match to: 849.5000, 931.5000
>P1;MVP_HUMAN
Major vault protein (MVP) (Lung resistance-related protein).- Homo sapiens (Human).
C;Species MVP_HUMAN: Homo sapiens (Human).
C;Family: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
C;Accession: Q14764; Q96BG4; Q9BPW6; Q9BQT1; Q9UBD1;
C;EMBL; X79882; CAA56256.2; -; mRNA.
C;EMBL; BC015623; AAH15623.1; -; mRNA.
C;EMBL; AJ238512; CAB55354.1; -; Genomic_DNA.
C;EMBL; AJ238514; CAB55354.1; JOINED; Genomic_DNA.
C;EMBL; AJ238516; CAB55354.1; JOINED; Genomic_DNA.
C;EMBL; AJ238518; CAB55354.1; JOINED; Genomic_DNA.
C;EMBL; AJ238519; CAB55355.1; -; Genomic_DNA.
C;EMBL; AJ238514; CAB55355.1; JOINED; Genomic_DNA.
C;EMBL; AJ238516; CAB55355.1; JOINED; Genomic_DNA.
C;EMBL; AJ238518; CAB55355.1; JOINED; Genomic_DNA.
C;EMBL; AJ291365; CAC35313.1; -; Genomic_DNA.
C;EMBL; AJ291366; CAC35314.1; -; mRNA.
C;EMBL; AJ291367; CAC35316.1; -; mRNA.
C;SRCDB SWISSPROT
C;--------------------------------------------------------------------------
C; The annotations (references, comments, and feature table) for this
C; SwissProt entry have been removed because of licensing restrictions.
C; See http://www.isb-sib.ch/announce/ for further information.
C; Academic, non-profit, and licensed users can retrieve the complete entry using this link:
C; http://www.expasy.ch/cgi-bin/get-sprot-entry?MVP_HUMAN
C;--------------------------------------------------------------------------
Đáy của Biểu mẫu
Mascot: http://www.matrixscience.com/
|
Chia sẻ với bạn bè của bạn: |