Đánh giá đa dạng di truyền nguồn gen Lilium spp bản địa và nhập nội bằng chỉ thị rapd nguyen Thi Phuong Thao, Ninh Thi Thao, Vu Quang Khanh, Nguyen Quang Thach



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Fig. 1. RAPD patterns generated by primer OPA-10 . Lanes M: 1 kb size marker; 1-32: L. oriental “Nostalgia”; L. longiflorum 1;L. formolongo; L. poilanei Gagn.; L. longiflorum 2; L. longiflorum 3; L. oriental hybrid “Cassandra”; L. oriental “Sorbonne”; L. oriental - trumpet “Gradisca”; L. oriental “Acapulco”; L. hybrid “Leslie”; L. oriental “Tiber”; L. oriental hybrid “Siberia”; L. oriental “Stargazer”; L. longiflorum “Deliana”; L. oriental “Crystal Star”; L. asiatic hybrid “Royal Show” ; L. longiflorum 4; L. oriental “Giacondo”; L. oriental “Mondriann”; L. oriental “Valadores”; L. oriental hybrid “Salinas”; L. oriental hybrid “Shalloon”; L. asiatic; L. brownii; L.asiatic hybrid “Freya”; L. asiatic hybrid “Quinta”; L. longiflorum 5; L. longiflorum “Georgia”; L. longiflorum “Snow Queen”; L. formosanum;L. speciosum

Fig.2. Dendrogram obtained from RAPD pattern of 27 Lilium accessions

4. CONCLUSION

Obviously with the ever-increasing production of new cultivars, the importance of accurate tools for assessing the genetic background and species or cultivar genuineness has become more important.

The RAPD technique provides a useful approach for evaluating genetic differentiation, particularly in Lilium species, which is still poorly known genetically. In this work, RAPD analysis combined with the construction of phylogenetic tree revealed the genetic variation and genetic relationship between the local and exotic lily cultivars and certain wild lily species from Vietnam. However, more efficient primers will be required to bring more reliable information which will asset the breeding program for Vietnam its self using valuable indigenous germplasm such as L. poilanei and L. aboricola.

Acknowledgements

This research was supported by fundamental scientific research in life science program code number 6 006 08 (2006-2008) from the Ministry of Science and Technology.

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