3. Results
3.1. Gas Production Kinetics Parameters
The effects of AOAN supplementation on the gas production kinetics parameters of
the TMR and the three different roughages are shown in Figure
1
. The gas production
and A at 48 h in the TMR, corn silage, and alfalfa hay were significantly increased by
AOAN supplementation (p < 0.05). The A of oat hay was increased by AOAN supple-
mentation (p < 0.05). Interestingly, the AGPR of TMR and corn silage increased by AOAN
supplementation (p < 0.05).
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To assess the correlation between the phenotypic variables and the relative abun-
dance of microbial genera, the Spearman correlation test was performed using SPSS 20.0
(IBM, Armonk, NY, USA), and plotted using GraphPad Prism 7 (GraphPad Software, San
Diego, CA, USA). For each correlation, the correlation coefficient value ranged from −1 to
+1 with larger absolute values indicating a stronger relationship and positive/negative
values indicating the direction of the association.
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